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KMID : 1007520110200010201
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Food Science and Biotechnology
2011 Volume.20 No. 1 p.201 ~ p.207
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Rapid and Simple Biochemical Detection for Salmonella spp. Using Modified LB Broth and the MUCAP Test
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Jang Keum-Il
Ahn Jun-Bae
Kim Kwang-Yup
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Abstract
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In this study, we developed a rapid Salmonella detection system and confirmed its detection efficiency using modified selective pre-enrichment and the 4-methyl umbelliferyl caprilate (MUCAP) test. Luria-Bertani (LB) medium without auto-fluorescence was modified by the addition of a bile salt mixture and sodium citrate as inhibitors, after which ferrous sulfate and sodium thiosulfate were added for the induction of H2S gas. In a mixed culture of Staphylococcus aureus, Escherichia coli, and Salmonella enteritidis in Salmonella detective modified LB (SDMLB) medium, S. enteritidis dominated regardless of their initial population size. Among 10 bacterial strains, Salmonella and Enterobacter spp. were cultured and distinguished by the H2S test because the Enterobacter spp. was unable to produce H2S gas in SDMLB medium. MUCAP testing produced blue fluorescence only for Salmonella spp. Tests for Salmonella using chicken skin and egg shells revealed the presence of Salmonella spp. Thus, foods carrying Salmonellae were detected rapidly and simply using this method.
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KEYWORD
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Salmonella, H2S test, 4-methyl umbelliferyl caprilate (MUCAP) test, Salmonella detective modified LB (SDMLB) medium, rapid detection
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